Last Name

Alicia Byrd

InstitutionUniversity of Arkansas for Medical Sciences
DepartmentBiochemistry & Molecular Biology, College of Medicine
DivisionBiochemistry & Molecular DNA & RNA
Address433-2 Biomedical Research II
200 South Cedar
Mail Slot # 516
Little Rock AR 72202
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    The major focus of my research in the Raney lab is helicase enzymology. Helicases are enzymes that remove secondary structures from DNA and RNA. We study two DNA helicases, Dda, from Bacteriophage T4, and a eukaryotic helicase, Pif1. In addition to unwinding duplex DNA, both helicases are known to displace proteins from DNA, and Pif1 unfolds an unusual four stranded DNA structures called a G-quadruplex. We also study a number of RNA helicases which we found bind tightly to G-quadruplex DNA. We use a number of methods to study helicases including equilibrium binding, ensemble kinetics (both pre-steady state and steady state), and single molecule kinetics (single molecule total internal reflection fluorescence microscopy).

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    American Chemical Society
    American Association for the Advancement of Science

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    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Byrd AK, Raney KD. Structure and function of Pif1 helicase. Biochem Soc Trans. 2017 Oct 15; 45(5):1159-1171. PMID: 28900015.
      View in: PubMed
    2. Griffin WC, Gao J, Byrd AK, Chib S, Raney KD. A biochemical and biophysical model of G-quadruplex DNA recognition by positive coactivator of transcription 4. J Biol Chem. 2017 06 09; 292(23):9567-9582. PMID: 28416612.
      View in: PubMed
    3. Byrd AK, Zybailov BL, Maddukuri L, Gao J, Marecki JC, Jaiswal M, Bell MR, Griffin WC, Reed MR, Chib S, Mackintosh SG, MacNicol AM, Baldini G, Eoff RL, Raney KD. Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress. J Biol Chem. 2016 08 19; 291(34):18041-57. PMID: 27369081; PMCID: PMC5016190 [Available on 08/19/17].
    4. Zybailov BL, Byrd AK, Glazko GV, Rahmatallah Y, Raney KD. Protein-protein interaction analysis for functional characterization of helicases. Methods. 2016 Oct 01; 108:56-64. PMID: 27090004; PMCID: PMC5035562 [Available on 10/01/17].
    5. Yu C, Tan HY, Choi M, Stanenas AJ, Byrd AK, D Raney K, Cohan CS, Bianco PR. SSB binds to the RecG and PriA helicases in vivo in the absence of DNA. Genes Cells. 2016 Feb; 21(2):163-84. PMID: 26766785; PMCID: PMC4738028 [Available on 02/01/17].
    6. Chib S, Byrd AK, Raney KD. Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes. J Biol Chem. 2016 Mar 11; 291(11):5889-901. PMID: 26733194; PMCID: PMC4786723 [Available on 03/11/17].
    7. Zybailov B, Gokulan K, Wiese J, Ramanagoudr-Bhojappa R, Byrd AK, Glazko G, Jaiswal M, Mackintosh S, Varughese KI, Raney KD. Analysis of Protein-protein Interaction Interface between Yeast Mitochondrial Proteins Rim1 and Pif1 Using Chemical Cross-linking Mass Spectrometry. J Proteomics Bioinform. 2015 Nov; 8(11):243-252. PMID: 26807012.
      View in: PubMed
    8. Byrd AK, Raney KD. Fine tuning of a DNA fork by the RecQ helicase. Proc Natl Acad Sci U S A. 2015 Dec 15; 112(50):15263-4. PMID: 26582797; PMCID: PMC4687568.
    9. Gao J, Zybailov BL, Byrd AK, Griffin WC, Chib S, Mackintosh SG, Tackett AJ, Raney KD. Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA. Chem Commun (Camb). 2015 Apr 28; 51(33):7242-4. PMID: 25813861; PMCID: PMC4465349.
    10. Byrd AK, Raney KD. A parallel quadruplex DNA is bound tightly but unfolded slowly by pif1 helicase. J Biol Chem. 2015 Mar 06; 290(10):6482-94. PMID: 25589786; PMCID: PMC4358282.
    11. Ramanagoudr-Bhojappa R, Byrd AK, Dahl C, Raney KD. Yeast Pif1 accelerates annealing of complementary DNA strands. Biochemistry. 2014 Dec 09; 53(48):7659-69. PMID: 25393406; PMCID: PMC4263423.
    12. Aarattuthodiyil S, Byrd AK, Raney KD. Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase. Nucleic Acids Res. 2014 Oct; 42(18):11707-20. PMID: 25249618; PMCID: PMC4191417.
    13. Waldrip ZJ, Byrum SD, Storey AJ, Gao J, Byrd AK, Mackintosh SG, Wahls WP, Taverna SD, Raney KD, Tackett AJ. A CRISPR-based approach for proteomic analysis of a single genomic locus. Epigenetics. 2014 Sep; 9(9):1207-11. PMID: 25147920; PMCID: PMC4169012.
    14. Mercado CP, Quintero MV, Li Y, Singh P, Byrd AK, Talabnin K, Ishihara M, Azadi P, Rusch NJ, Kuberan B, Maroteaux L, Kilic F. A serotonin-induced N-glycan switch regulates platelet aggregation. Sci Rep. 2013 Sep 30; 3:2795. PMID: 24077408; PMCID: PMC3786303.
    15. Ramanagoudr-Bhojappa R, Chib S, Byrd AK, Aarattuthodiyil S, Pandey M, Patel SS, Raney KD. Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA. J Biol Chem. 2013 May 31; 288(22):16185-95. PMID: 23596008; PMCID: PMC3668774.
    16. Raney KD, Byrd AK, Aarattuthodiyil S. Structure and Mechanisms of SF1 DNA Helicases. Adv Exp Med Biol. 2013; 973:E1. PMID: 23269543.
      View in: PubMed
    17. Raney KD, Byrd AK, Aarattuthodiyil S. Structure and Mechanisms of SF1 DNA Helicases. Adv Exp Med Biol. 2013; 767:17-46. PMID: 23161005; PMCID: PMC3806203.
    18. Byrd AK, Raney KD. Superfamily 2 helicases. Front Biosci (Landmark Ed). 2012 Jun 01; 17:2070-88. PMID: 22652765; PMCID: PMC3775597.
    19. He X, Byrd AK, Yun MK, Pemble CW, Harrison D, Yeruva L, Dahl C, Kreuzer KN, Raney KD, White SW. The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. Structure. 2012 Jul 03; 20(7):1189-200. PMID: 22658750; PMCID: PMC3392491.
    20. Byrd AK, Matlock DL, Bagchi D, Aarattuthodiyil S, Harrison D, Croquette V, Raney KD. Dda helicase tightly couples translocation on single-stranded DNA to unwinding of duplex DNA: Dda is an optimally active helicase. J Mol Biol. 2012 Jul 13; 420(3):141-54. PMID: 22504228; PMCID: PMC3372602.
    21. Liu J, Choi M, Stanenas AG, Byrd AK, Raney KD, Cohan C, Bianco PR. Novel, fluorescent, SSB protein chimeras with broad utility. Protein Sci. 2011 Jun; 20(6):1005-20. PMID: 21462278; PMCID: PMC3104230.
    22. Matlock DL, Yeruva L, Byrd AK, Mackintosh SG, Langston C, Brown C, Cameron CE, Fischer CJ, Raney KD. Investigation of translocation, DNA unwinding, and protein displacement by NS3h, the helicase domain from the hepatitis C virus helicase. Biochemistry. 2010 Mar 16; 49(10):2097-109. PMID: 20108974; PMCID: PMC2853022.
    23. Byrd AK, Raney KD. Displacement of a DNA binding protein by Dda helicase. Nucleic Acids Res. 2006; 34(10):3020-9. PMID: 16738140; PMCID: PMC1474059.
    24. Byrd AK, Raney KD. Increasing the length of the single-stranded overhang enhances unwinding of duplex DNA by bacteriophage T4 Dda helicase. Biochemistry. 2005 Oct 04; 44(39):12990-7. PMID: 16185067.
      View in: PubMed
    25. Byrd AK, Raney KD. Protein displacement by an assembly of helicase molecules aligned along single-stranded DNA. Nat Struct Mol Biol. 2004 Jun; 11(6):531-8. PMID: 15146172.
      View in: PubMed
    26. Ziemba A, Reed M, Raney KD, Byrd AB, Ebbinghaus S. A bis-conjugated TFO inhibits target gene expression intracellularly and prevents triplex unwinding due to helicase activity. Biochemistry. 2003; 42(17):5013-5024.
    27. Nanduri B, Byrd AK, Eoff RL, Tackett AJ, Raney KD. Pre-steady-state DNA unwinding by bacteriophage T4 Dda helicase reveals a monomeric molecular motor. Proc Natl Acad Sci U S A. 2002 Nov 12; 99(23):14722-7. PMID: 12411580; PMCID: PMC137486.
    28. Morris PD, Byrd AK, Tackett AJ, Cameron CE, Tanega P, Ott R, Fanning E, Raney KD. Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA. Biochemistry. 2002 Feb 19; 41(7):2372-8. PMID: 11841230.
      View in: PubMed
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