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Vladimir Lupashin

TitleProfessor
InstitutionUniversity of Arkansas for Medical Sciences
DepartmentPhysiology & Cell Biology, College of Medicine
Address261-2 Biomedical Research II
200 South Cedar
Mail Slot # 505
Little Rock AR 72202
Phone501-603-1170
ORCID ORCID Icon0000-0002-2350-1962 Additional info
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    Collapse Overview 
    Collapse overview
    Our laboratory is interested in understanding the molecular mechanisms responsible for the generation and maintenance of intra-cellular membrane-bounded compartments. In all eukaryotic cells intracellular membrane trafficking is critical for a range of important cellular functions including protein secretion, post-translational modifications, cell signalling, cell polarization, and cell maintenance. Defects in membrane trafficking can underline, or even exacerbate, a number of human diseases including cancer, diabetes mellitus, Alzheimer’s, cystic fibrosis, Hermansky-Pudlak syndrome and Congenital Disorders of Glycosylation.
    Our research directed towards the understanding of the basic mechanisms of intracellular vesicular trafficking using both yeast and mammalian tissue culture cell model systems. Our lab played a principal role in the discovery of a novel vesicle tethering factors, published more than 60 original papers in high-profile journals, including Journal of Cell Biology, PNAS, Science, Journal of Neuroscience, Molecular Biology of Cell and Nature Communications. My current research has been continuously supported by grants from both NSF and NIH.
    We have pioneered the functional analysis of the Conserved Oligomeric Golgi (COG), an evolutionarily conserved complex of eight gene products, each of which is critical for the membrane trafficking and protein modifications in the Golgi apparatus. The COG complex interacts with core fusion machinery components including SNAREs, SM proteins, Rabs, coiled-coil tethers and COPI coat to organize specific docking and fusion of transport intermediates with their acceptor membrane. By using state of the art biochemical, genetic and microscopy approaches (including mass-spectrometry, electron and super-resolution microscopy, CRISPR directed gene editing techniques) we would like to determine how the key components of intracellular membrane trafficking machinery work together to direct efficient protein trafficking in human cells in health and disease.


    Collapse Affiliation 
    Collapse member of
    American Society for Cell Biology
    American Society for Biochemistry and Molecular Biology
    Society for Glycobiology

    Collapse Research 
    Collapse research activities and funding
    2R01GM083144     (LUPASHIN, VLADIMIR V)Apr 1, 2023 - Mar 31, 2027
    NIH/National Institutes of Health
    Characterization of mammalian COG complex-interacting Golgi trafficking machinery
    Role: Principal Investigator
    2R01GM083144-14A1     (LUPASHIN, VLADIMIR V)Apr 1, 2023 - Mar 31, 2027
    NIH/National Institutes of Health
    Characterization of mammalian COG complex-interacting Golgi trafficking machinery
    Role: Principal Investigator
    1R01AI158372     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    1R01AI158372-01A1     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    R01AI158372     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    R01AI158372-03     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    R5 01AI158372-04     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    R501AI158372     (LUPASHIN, VLADIMIR V)Apr 22, 2022 - Mar 31, 2027
    NIH/National Institutes of Health - Pass Through: Washington State University
    Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub
    Role: Principal Investigator
    Research Council FY2018     (LUPASHIN, VLADIMIR V)Dec 1, 2017 - Dec 31, 2018
    UAMS Internal Research Awards
    Characterization of Mammalian COG Complex-Interacting Golgi Trafficking Machinery
    Role: Principal Investigator
    ResearchCouncilFY2018     (LUPASHIN, VLADIMIR V)Dec 1, 2017 - Dec 31, 2018
    UAMS Internal Research Awards
    Characterization of Mammalian COG Complex-Interacting Golgi Trafficking Machinery
    Role: Principal Investigator
    R01AI129992     (LUPASHIN, VLADIMIR V)Jan 5, 2017 - Jun 30, 2020
    NIH/Nat. Inst. of General Medical Sciences - Pass Through: Washington State University
    Modulation of host secretory trafficking by the Brucella effector BspB - Continuation - Continuation
    Role: Principal Investigator
    Lupashin Retension package     (LUPASHIN, VLADIMIR V)Sep 4, 2015 - Jun 30, 2016
    UAMS College of Medicine
    COM-Intramural Award
    Role: Principal Investigator
    LupashinRetensionpackage     (LUPASHIN, VLADIMIR V)Sep 4, 2015 - Jun 30, 2016
    UAMS College of Medicine
    COM-Intramural Award
    Role: Principal Investigator
    S10OD018065     (STORRIE, BRIAN)Jul 1, 2015 - Jun 30, 2017
    NIH
    Super-Resolution Light Microscope at University of Arkansas for Medical Sciences
    Role: Co-Investigator
    U54GM105814     (STORRIE, BRIAN)Aug 1, 2013 - Oct 31, 2018
    NIH
    Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function
    Role: Co-Principal Investigator
    DBI-0959745     (STORRIE, BRIAN)Jan 15, 2010 - Dec 31, 2012
    National Science Foundation
    120 kV FEI Electron Microscope and Supporting Sample Preparation Equipment for Biological Microscopy
    Role: Co-Investigator
    R01GM083144     (LUPASHIN, VLADIMIR V)Aug 1, 2008 - Mar 31, 2027
    NIH
    Characterization of mammalian COG complex-interacting Golgi trafficking machinery
    Role: Principal Investigator
    MCB     (LUPASHIN, VLADIMIR V)Apr 1, 2007 - Sep 30, 2011
    National Science Foundation
    Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast
    Role: Principal Investigator
    MCB-0645163     (LUPASHIN, VLADIMIR V)Apr 1, 2007 - Sep 30, 2011
    National Science Foundation
    Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast
    Role: Principal Investigator
    S3     (LUPASHIN, VLADIMIR V)Apr 1, 2007 - Sep 30, 2011
    National Science Foundation
    Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast
    Role: Principal Investigator
    S3 - Correct     (LUPASHIN, VLADIMIR V)Apr 1, 2007 - Sep 30, 2011
    National Science Foundation
    Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast
    Role: Principal Investigator
    S3-Correct     (LUPASHIN, VLADIMIR V)Apr 1, 2007 - Sep 30, 2011
    National Science Foundation
    Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast
    Role: Principal Investigator
    060082     (LUPASHIN, VLADIMIR)Apr 1, 2006 - Mar 31, 2007
    Mizutani Foundation for Glycoscience
    Role of COG complex mediated vesicle tethering is localization of Golgi enzymes
    Role: Principal Investigator
    60082     (LUPASHIN, VLADIMIR)Apr 1, 2006 - Mar 31, 2007
    Mizutani Foundation for Glycoscience
    Role of COG complex mediated vesicle tethering is localization of Golgi enzymes
    Role: Principal Investigator
    MCB0234822     (LUPASHIN, VLADIMIR)Jan 1, 2003 - Dec 31, 2006
    National Science Foundation
    Structural and functional analysis of SEC 34 Protein complex
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions. Don't see publications published under other names? Login to add alternative names.
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    1. Marsilia C, Batra M, Pokrovskaya ID, Wang C, Chaput D, Naumova DA, Lupashin VV, Suvorova ES. Essential role of the conserved oligomeric Golgi complex in Toxoplasma gondii. mBio. 2023 Nov 15; e0251323. PMID: 37966241.
      View in: PubMed
    2. Duan R, Marafi D, Xia ZJ, Ng BG, Maroofian R, Sumya FT, Saad AK, Du H, Fatih JM, Hunter JV, Elbendary HM, Baig SM, Abdullah U, Ali Z, Efthymiou S, Murphy D, Mitani T, Withers MA, Jhangiani SN, Coban-Akdemir Z, Calame DG, Pehlivan D, Gibbs RA, Posey JE, Houlden H, Lupashin VV, Zaki MS, Freeze HH, Lupski JR. Biallelic missense variants in COG3 cause a congenital disorder of glycosylation with impairment of retrograde vesicular trafficking. J Inherit Metab Dis. 2023 11; 46(6):1195-1205. PMID: 37711075.
      View in: PubMed
    3. D'Souza Z, Pokrovskaya I, Lupashin VV. Syntaxin-5's flexibility in SNARE pairing supports Golgi functions. Traffic. 2023 08; 24(8):355-379. PMID: 37340984.
      View in: PubMed
    4. Dimori M, Pokrovskaya ID, Liu S, Sherrill JT, Gomez-Acevedo H, Fu Q, Storrie B, Lupashin VV, Morello R. A Rab33b missense mouse model for Smith-McCort dysplasia shows bone resorption defects and altered protein glycosylation. Front Genet. 2023; 14:1204296. PMID: 37359363.
      View in: PubMed
    5. Vicogne D, Beauval N, Durin Z, Allorge D, Kondratska K, Haustrate A, Prevarskaya N, Lupashin V, Legrand D, Foulquier F. Insights into the regulation of cellular Mn2+ homeostasis via TMEM165. Biochim Biophys Acta Mol Basis Dis. 2023 08; 1869(6):166717. PMID: 37062452.
      View in: PubMed
    6. Khakurel A, Lupashin VV. Role of GARP Vesicle Tethering Complex in Golgi Physiology. Int J Mol Sci. 2023 Mar 23; 24(7). PMID: 37047041.
      View in: PubMed
    7. Wang Y, Lupashin VV, Graham TR. Correction to: Golgi. Methods Mol Biol. 2023; 2557:C1. PMID: 36763332.
      View in: PubMed
    8. Sumya FT, Pokrovskaya ID, Lupashin VV. Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System. Methods Mol Biol. 2023; 2557:365-390. PMID: 36512227.
      View in: PubMed
    9. Khakurel A, Kudlyk T, Lupashin VV. Generation and Analysis of hTERT-RPE1 VPS54 Knock-Out and Rescued Cell Lines. Methods Mol Biol. 2023; 2557:349-364. PMID: 36512226.
      View in: PubMed
    10. Sumya FT, Pokrovskaya ID, D'Souza Z, Lupashin VV. Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra-Golgi recycling vesicles. Traffic. 2023 Feb; 24(2):52-75. PMID: 36468177.
      View in: PubMed
    11. Khakurel A, Kudlyk T, Pokrovskaya I, D'Souza Z, Lupashin VV. GARP dysfunction results in COPI displacement, depletion of Golgi v-SNAREs and calcium homeostasis proteins. Front Cell Dev Biol. 2022; 10:1066504. PMID: 36578782.
      View in: PubMed
    12. D'Souza Z, Sumya FT, Khakurel A, Lupashin V. Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation. Cells. 2021 11 23; 10(12). PMID: 34943782.
      View in: PubMed
    13. Sumya FT, Pokrovskaya ID, Lupashin V. Development and Initial Characterization of Cellular Models for COG Complex-Related CDG-II Diseases. Front Genet. 2021; 12:733048. PMID: 34603392.
      View in: PubMed
    14. Khakurel A, Kudlyk T, Bonifacino JS, Lupashin VV. The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery. Mol Biol Cell. 2021 08 15; 32(17):1594-1610. PMID: 34161137.
      View in: PubMed
    15. Adusumalli R, ?sheim HC, Lupashin V, Blackburn JB, Prydz K. Proteoglycan synthesis in conserved oligomeric Golgi subunit deficient HEK293T cells is affected differently, depending on the lacking subunit. Traffic. 2021 07; 22(7):230-239. PMID: 34053170.
      View in: PubMed
    16. Hartwig C, M?ndez GM, Bhattacharjee S, Vrailas-Mortimer AD, Zlatic SA, Freeman AAH, Gokhale A, Concilli M, Werner E, Sapp Savas C, Rudin-Rush S, Palmer L, Shearing N, Margewich L, McArthy J, Taylor S, Roberts B, Lupashin V, Polishchuk RS, Cox DN, Jorquera RA, Faundez V. Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content. J Neurosci. 2021 01 13; 41(2):215-233. PMID: 33208468.
      View in: PubMed
    17. Petitjean O, Girardi E, Ngondo RP, Lupashin V, Pfeffer S. Genome-Wide CRISPR-Cas9 Screen Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection. mSphere. 2020 11 11; 5(6). PMID: 33177215.
      View in: PubMed
    18. D'Souza Z, Taher FS, Lupashin VV. Golgi inCOGnito: From vesicle tethering to human disease. Biochim Biophys Acta Gen Subj. 2020 11; 1864(11):129694. PMID: 32730773.
      View in: PubMed
    19. Murali P, Johnson BP, Lu Z, Climer L, Scott DA, Foulquier F, Oprea-Ilies G, Lupashin V, Drake RR, Abbott KL. Novel role for the Golgi membrane protein TMEM165 in control of migration and invasion for breast carcinoma. Oncotarget. 2020 Jul 14; 11(28):2747-2762. PMID: 32733646.
      View in: PubMed
    20. Realegeno S, Priyamvada L, Kumar A, Blackburn JB, Hartloge C, Puschnik AS, Sambhara S, Olson VA, Carette JE, Lupashin V, Satheshkumar PS. Conserved Oligomeric Golgi (COG) Complex Proteins Facilitate Orthopoxvirus Entry, Fusion and Spread. Viruses. 2020 06 30; 12(7). PMID: 32629851.
      View in: PubMed
    21. Prydz K, Lupashin V, Wang Y, Saraste J. Editorial: Golgi Dynamics in Physiological and Pathological Conditions. Front Cell Dev Biol. 2020; 8:7. PMID: 32064258.
      View in: PubMed
    22. Vicogne D, Houdou M, Garat A, Climer L, Lupashin V, Morelle W, Foulquier F. Fetal bovine serum impacts the observed N-glycosylation defects in TMEM165 KO HEK cells. J Inherit Metab Dis. 2020 03; 43(2):357-366. PMID: 31415112.
      View in: PubMed
    23. Li Y, Hadden C, Cooper A, Ahmed A, Wu H, Lupashin VV, Mayeux PR, Kilic F. Retraction Note: Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability. Sci Rep. 2019 Sep 27; 9(1):14218. PMID: 31562363.
      View in: PubMed
    24. Blackburn JB, D'Souza Z, Lupashin VV. Maintaining order: COG complex controls Golgi trafficking, processing, and sorting. FEBS Lett. 2019 09; 593(17):2466-2487. PMID: 31381138.
      View in: PubMed
    25. Lebredonchel E, Houdou M, Potelle S, de Bettignies G, Schulz C, Krzewinski Recchi MA, Lupashin V, Legrand D, Klein A, Foulquier F. Dissection of TMEM165 function in Golgi glycosylation and its Mn2+ sensitivity. Biochimie. 2019 Oct; 165:123-130. PMID: 31351090.
      View in: PubMed
    26. D'Souza Z, Blackburn JB, Kudlyk T, Pokrovskaya ID, Lupashin VV. Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells. Front Cell Dev Biol. 2019; 7:118. PMID: 31334232.
      View in: PubMed
    27. Ahmed BA, Jeffus BC, Bukhari SIA, Harney JT, Unal R, Lupashin VV, van der Sluijs P, Kilic F. Withdrawal: Serotonin transamidates Rab4 and facilitates its binding to the C terminus of serotonin transporter. J Biol Chem. 2019 Jun 14; 294(24):9658. PMID: 31201246.
      View in: PubMed
    28. Ahmed BA, Jeffus BC, Bukhari SIA, Harney JT, Unal R, Lupashin VV, van der Sluijs P, Kilic F. Expression of Concern: Serotonin transamidates Rab4 and facilitates its binding to the C terminus of serotonin transporter. J Biol Chem. 2019 03 29; 294(13):5209. PMID: 30926757.
      View in: PubMed
    29. Ishii M, Lupashin VV, Nakano A. Detailed Analysis of the Interaction of Yeast COG Complex. Cell Struct Funct. 2018 Jul 19; 43(2):119-127. PMID: 29899178.
      View in: PubMed
    30. Blackburn JB, Kudlyk T, Pokrovskaya I, Lupashin VV. More than just sugars: Conserved oligomeric Golgi complex deficiency causes glycosylation-independent cellular defects. Traffic. 2018 06; 19(6):463-480. PMID: 29573151.
      View in: PubMed
    31. Climer LK, Pokrovskaya ID, Blackburn JB, Lupashin VV. Membrane detachment is not essential for COG complex function. Mol Biol Cell. 2018 04 15; 29(8):964-974. PMID: 29467253.
      View in: PubMed
    32. Climer LK, Hendrix RD, Lupashin VV. Conserved Oligomeric Golgi and Neuronal Vesicular Trafficking. Handb Exp Pharmacol. 2018; 245:227-247. PMID: 29063274.
      View in: PubMed
    33. Miller CN, Smith EP, Cundiff JA, Knodler LA, Bailey Blackburn J, Lupashin V, Celli J. A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication. Cell Host Microbe. 2017 Sep 13; 22(3):317-329.e7. PMID: 28844886.
      View in: PubMed
    34. Potelle S, Dulary E, Climer L, Duvet S, Morelle W, Vicogne D, Lebredonchel E, Houdou M, Spriet C, Krzewinski-Recchi MA, Peanne R, Klein A, de Bettignies G, Morsomme P, Matthijs G, Marquardt T, Lupashin V, Foulquier F. Manganese-induced turnover of TMEM165. Biochem J. 2017 04 19; 474(9):1481-1493. PMID: 28270545.
      View in: PubMed
    35. Hadden C, Fahmi T, Cooper A, Savenka AV, Lupashin VV, Roberts DJ, Maroteaux L, Hauguel-de Mouzon S, Kilic F. Serotonin transporter protects the placental cells against apoptosis in caspase 3-independent pathway. J Cell Physiol. 2017 Dec; 232(12):3520-3529. PMID: 28109119.
      View in: PubMed
    36. Morelle W, Potelle S, Witters P, Wong S, Climer L, Lupashin V, Matthijs G, Gadomski T, Jaeken J, Cassiman D, Morava E, Foulquier F. Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects. J Clin Endocrinol Metab. 2017 Apr 01; 102(4):1375-1386. PMID: 28323990.
      View in: PubMed
    37. Comstra HS, McArthy J, Rudin-Rush S, Hartwig C, Gokhale A, Zlatic SA, Blackburn JB, Werner E, Petris M, D'Souza P, Panuwet P, Barr DB, Lupashin V, Vrailas-Mortimer A, Faundez V. The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors. Elife. 2017 03 29; 6. PMID: 28355134.
      View in: PubMed
    38. Siegel N, Lupashin V, Storrie B, Brooker G. High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers. Nat Photonics. 2016 Dec; 10:802-808. PMID: 28261321.
      View in: PubMed
    39. Willett R, Blackburn JB, Climer L, Pokrovskaya I, Kudlyk T, Wang W, Lupashin V. COG lobe B sub-complex engages v-SNARE GS15 and functions via regulated interaction with lobe A sub-complex. Sci Rep. 2016 07 07; 6:29139. PMID: 27385402.
      View in: PubMed
    40. Bailey Blackburn J, Pokrovskaya I, Fisher P, Ungar D, Lupashin VV. COG Complex Complexities: Detailed Characterization of a Complete Set of HEK293T Cells Lacking Individual COG Subunits. Front Cell Dev Biol. 2016; 4:23. PMID: 27066481.
      View in: PubMed
    41. Li Y, Hadden C, Cooper A, Ahmed A, Wu H, Lupashin VV, Mayeux PR, Kilic F. Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability. Sci Rep. 2016 Mar 09; 6:22747. PMID: 26956613.
      View in: PubMed
    42. Liu S, Majeed W, Kudlyk T, Lupashin V, Storrie B. Identification of Rab41/6d Effectors Provides an Explanation for the Differential Effects of Rab41/6d and Rab6a/a' on Golgi Organization. Front Cell Dev Biol. 2016; 4:13. PMID: 26973836.
      View in: PubMed
    43. Blackburn JB, Lupashin VV. Creating Knockouts of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function. Methods Mol Biol. 2016; 1496:145-61. PMID: 27632008.
      View in: PubMed
    44. Climer LK, Dobretsov M, Lupashin V. Defects in the COG complex and COG-related trafficking regulators affect neuronal Golgi function. Front Neurosci. 2015; 9:405. PMID: 26578865.
      View in: PubMed
    45. Willett RA, Kudlyk TA, Lupashin VV. Expression of functional Myc-tagged conserved oligomeric Golgi (COG) subcomplexes in mammalian cells. Methods Mol Biol. 2015; 1270:167-77. PMID: 25702117.
      View in: PubMed
    46. Ha JY, Pokrovskaya ID, Climer LK, Shimamura GR, Kudlyk T, Jeffrey PD, Lupashin VV, Hughson FM. Cog5-Cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex. Proc Natl Acad Sci U S A. 2014 Nov 04; 111(44):15762-7. PMID: 25331899.
      View in: PubMed
    47. Zhang Y, Yeruva L, Marinov A, Prantner D, Wyrick PB, Lupashin V, Nagarajan UM. The DNA sensor, cyclic GMP-AMP synthase, is essential for induction of IFN-? during Chlamydia trachomatis infection. J Immunol. 2014 Sep 01; 193(5):2394-404. PMID: 25070851.
      View in: PubMed
    48. Willett R, Pokrovskaya I, Kudlyk T, Lupashin V. Multipronged interaction of the COG complex with intracellular membranes. Cell Logist. 2014 Jan 01; 4(1):e27888. PMID: 24649395.
      View in: PubMed
    49. Nishimura T, Uchida Y, Yachi R, Kudlyk T, Lupashin V, Inoue T, Taguchi T, Arai H. Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs. Mol Biol Cell. 2013 Nov; 24(22):3534-44. PMID: 24048449.
      View in: PubMed
    50. Willett R, Ungar D, Lupashin V. The Golgi puppet master: COG complex at center stage of membrane trafficking interactions. Histochem Cell Biol. 2013 Sep; 140(3):271-83. PMID: 23839779.
      View in: PubMed
    51. Willett R, Kudlyk T, Pokrovskaya I, Sch?nherr R, Ungar D, Duden R, Lupashin V. COG complexes form spatial landmarks for distinct SNARE complexes. Nat Commun. 2013; 4:1553. PMID: 23462996.
      View in: PubMed
    52. Willett RA, Pokrovskaya ID, Lupashin VV. Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of Golgi glycosyltransferases in COG complex depleted mammalian cells. Methods Mol Biol. 2013; 1022:61-72. PMID: 23765654.
      View in: PubMed
    53. Miller VJ, Sharma P, Kudlyk TA, Frost L, Rofe AP, Watson IJ, Duden R, Lowe M, Lupashin VV, Ungar D. Molecular insights into vesicle tethering at the Golgi by the conserved oligomeric Golgi (COG) complex and the golgin TATA element modulatory factor (TMF). J Biol Chem. 2013 Feb 08; 288(6):4229-40. PMID: 23239882.
      View in: PubMed
    54. Kudlyk T, Willett R, Pokrovskaya ID, Lupashin V. COG6 interacts with a subset of the Golgi SNAREs and is important for the Golgi complex integrity. Traffic. 2013 Feb; 14(2):194-204. PMID: 23057818.
      View in: PubMed
    55. Gokhale A, Larimore J, Werner E, So L, Moreno-De-Luca A, Lese-Martin C, Lupashin VV, Smith Y, Faundez V. Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1. J Neurosci. 2012 Mar 14; 32(11):3697-711. PMID: 22423091.
      View in: PubMed
    56. Pokrovskaya ID, Szwedo JW, Goodwin A, Lupashina TV, Nagarajan UM, Lupashin VV. Chlamydia trachomatis hijacks intra-Golgi COG complex-dependent vesicle trafficking pathway. Cell Microbiol. 2012 May; 14(5):656-68. PMID: 22233276.
      View in: PubMed
    57. Flanagan-Steet H, Johnson S, Smith RD, Bangiyeva J, Lupashin V, Steet R. Mislocalization of large ARF-GEFs as a potential mechanism for BFA resistance in COG-deficient cells. Exp Cell Res. 2011 Oct 01; 317(16):2342-52. PMID: 21722633.
      View in: PubMed
    58. Pokrovskaya ID, Willett R, Smith RD, Morelle W, Kudlyk T, Lupashin VV. Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery. Glycobiology. 2011 Dec; 21(12):1554-69. PMID: 21421995.
      View in: PubMed
    59. Sztul E, Lupashin V. Role of vesicle tethering factors in the ER-Golgi membrane traffic. FEBS Lett. 2009 Dec 03; 583(23):3770-83. PMID: 19887069.
      View in: PubMed
    60. Richardson BC, Smith RD, Ungar D, Nakamura A, Jeffrey PD, Lupashin VV, Hughson FM. Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene. Proc Natl Acad Sci U S A. 2009 Aug 11; 106(32):13329-34. PMID: 19651599.
      View in: PubMed
    61. Smith RD, Willett R, Kudlyk T, Pokrovskaya I, Paton AW, Paton JC, Lupashin VV. The COG complex, Rab6 and COPI define a novel Golgi retrograde trafficking pathway that is exploited by SubAB toxin. Traffic. 2009 Oct; 10(10):1502-17. PMID: 19678899.
      View in: PubMed
    62. Ahmed BA, Bukhari IA, Jeffus BC, Harney JT, Thyparambil S, Ziu E, Fraer M, Rusch NJ, Zimniak P, Lupashin V, Tang D, Kilic F. The cellular distribution of serotonin transporter is impeded on serotonin-altered vimentin network. PLoS One. 2009; 4(3):e4730. PMID: 19270731.
      View in: PubMed
    63. Smith RD, Lupashin VV. Role of the conserved oligomeric Golgi (COG) complex in protein glycosylation. Carbohydr Res. 2008 Aug 11; 343(12):2024-31. PMID: 18353293.
      View in: PubMed
    64. Smith R.D, Lupashin V.V. Mini-Review: Role of the Conserved Oligomeric Golgi (COG) complex in protein glycosylation. Carbohydrate Research. 2008; 343:2024-2031.
    65. Ahmed BA, Jeffus BC, Bukhari SI, Harney JT, Unal R, Lupashin VV, van der Sluijs P, Kilic F. Serotonin transamidates Rab4 and facilitates its binding to the C terminus of serotonin transporter. J Biol Chem. 2008 Apr 04; 283(14):9388-98. PMID: 18227069.
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    66. Lupashin VV, Ungar D. The Golgi Apparatus, State of the art 110 years after Camillo Golgi discovery. The COG complex. 2008.
    67. Smith RD, Lupashin V. The COG complex dynamically assembles on the Golgi membrane to regulate intra-Golgi trafficing. Febs J. 2008; (275):203.
    68. Lupashin VV, Sztul E. Protein Trafficking: Mechanisms and Regulations. Tethering factors. 2008.
    69. Ahmed BA, Jeffus BC, Bukhari SI, Harney JT, Unal R, Lupashin VV, Van der SP, Kilic F. Serotonin transamidates Rab4 and facilitates its binding to the C-terminus of SERT. J Biol Chem. 2008; 283(14):9388-9398.
    70. Lupashin V, Smith RD, Shestakova AM. The COG complex interacts with Syntaxin5a to stabilize intra-Golgi SNARE complex and to direct trafficking of recycling intra-Golgi vesicles. FASEB J. 2008; (22):8115-816.
    71. Shestakova A, Suvorova E, Pavliv O, Khaidakova G, Lupashin V. Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability. J Cell Biol. 2007 Dec 17; 179(6):1179-92. PMID: 18086915.
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    72. Sun Y, Shestakova A, Hunt L, Sehgal S, Lupashin V, Storrie B. Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis. Mol Biol Cell. 2007 Oct; 18(10):4129-42. PMID: 17699596.
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    73. Ng BG, Kranz C, Hagebeuk EE, Duran M, Abeling NG, Wuyts B, Ungar D, Lupashin V, Hartdorff CM, Poll-The BT, Freeze HH. Molecular and clinical characterization of a Moroccan Cog7 deficient patient. Mol Genet Metab. 2007 Jun; 91(2):201-4. PMID: 17395513.
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    74. Kranz C, Ng BG, Sun L, Sharma V, Eklund EA, Miura Y, Ungar D, Lupashin V, Winkel RD, Cipollo JF, Costello CE, Loh E, Hong W, Freeze HH. COG8 deficiency causes new congenital disorder of glycosylation type IIh. Hum Mol Genet. 2007 Apr 01; 16(7):731-41. PMID: 17331980.
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    75. Shestakova A, Smith R.D, Pavliv O, Khaidakova G, Lupashin V.V. The COG complex interacts with the t-SNARE Syntaxin5 to direct trafficking of recyling intra-Golgi vesicles that is essential for normal protein glycosylation. 2007.
    76. Shestakova A, Zolov S, Lupashin V. COG complex-mediated recycling of Golgi glycosyltransferases is essential for normal protein glycosylation. Traffic. 2006 Feb; 7(2):191-204. PMID: 16420527.
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    77. Sztul E, Lupashin V. Role of tethering factors in secretory membrane traffic. Am J Physiol Cell Physiol. 2006 Jan; 290(1):C11-26. PMID: 16338975.
      View in: PubMed
    78. Shestakova A, Suvorova E, Pavliv O, Khaidakova G, Lupashin V.V. The Conserved Oligomeric Golgi complex functional interaction with SNARE and Rab proteins. Imaging Membrane Dynamics. 2006.
    79. Shestakova A, Suvorova E, Pavliv O, Khaidakova G, Lupashin V.V. The Conserved Oligomeric Golgi complex directly interacts with the t-SNARE Syntaxin5/Sed5 and regulates formation of the intra-Golgi SNARE complex. 2006.
    80. Lupashin VV, Shestakova A, Zolov S. The COG Complex-mediated Docking of intra-Golgi Vesicles is Essential for Normal Protein Glycosylation. 2006.
    81. Lupashin V, Zolov S, Shestakova A, Fotso P. Conserved Oligomeric Complex Directs Docking of Intra-Golgi Retrograde Vesicles. 2006.
    82. Fotso P, Koryakina Y, Pavliv O, Tsiomenko AB, Lupashin VV. Cog1p plays a central role in the organization of the yeast conserved oligomeric Golgi complex. J Biol Chem. 2005 Jul 29; 280(30):27613-23. PMID: 15932880.
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    83. Lupashin V, Sztul E. Golgi tethering factors. Biochim Biophys Acta. 2005 Jul 10; 1744(3):325-39. PMID: 15979505.
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    84. Zolov SN, Lupashin VV. Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells. J Cell Biol. 2005 Feb 28; 168(5):747-59. PMID: 15728195.
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    85. Shestakova A, Zolov S, Lupashin V. The COG Complex-mediated Recyling of Golgi Enzymes is Essential for Normal Protein Glycosylation. 2005.
    86. Lupashin V.V, Zolov S.N, Shestakova A. The COG Complex regulates intra-Golgi cycling of vesicles that carry SNAREs and glycosyltransferases. 2005.
    87. Kilic F, Lupashin V, Van der Sluijs P. Serotonin Regulates the Plasma Membrane Expression of Serotonin Transporter Protein Via its Interactions with Rab4. 2005.
    88. Zolov S, Lupashin V. CCD vesicles dock to Golgi in vitro in a Cog3p-dependent reaction. 2005.
    89. Koriakina Y, Fotso P, Lupashin V.V. Cog1 is a bridging subunit between the two lobes of Conserved Oligomeric Golgi (COG) complex. Experimental Biology Meeting. 2005.
    90. Fotso P, Koriakina Y, Lupashin V.V. Cog1p plays a central role in the organization of the yeast Conserved Oligomeric Golgi (COG) complex. 2004.
    91. Fotso P.K, Lamm A, Lupashin V.V. Characterization of interactions between the subunits of the yeast Conserved Oligomeric Golgi (COG) complex. 2003; 105a-106a.
    92. Lupashin V.V, Suvorova E.S, Duden R. Yeast COG complex, a Ypt1 effector required for retrograde intra-Golgi trafficking COG interacts with the Golgi SNAREs and with COPI vesicle coat proteins. 2003.
    93. Suvorova ES, Duden R, Lupashin VV. The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins. J Cell Biol. 2002 May 13; 157(4):631-43. PMID: 12011112.
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    94. Ungar D, Oka T, Brittle EE, Vasile E, Lupashin VV, Chatterton JE, Heuser JE, Krieger M, Waters MG. Characterization of a mammalian Golgi-localized protein complex, COG, that is required for normal Golgi morphology and function. J Cell Biol. 2002 Apr 29; 157(3):405-15. PMID: 11980916.
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    95. Suvorova ES, Kurten RC, Lupashin VV. Identification of a human orthologue of Sec34p as a component of the cis-Golgi vesicle tethering machinery. J Biol Chem. 2001 Jun 22; 276(25):22810-8. PMID: 11292827.
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    96. Suvorova E.S, Lupashin V.V. Sec34p complex interacts with key components of intra-Golgi trafficking machinery. 2001; 12S: 210a.
    97. Eddington A.D, Shank B.B, Lupashin V.V, Kurten R.C. Sorting Nexin complex formation by PX domain is necessary for endosome binding. 2001; 12S: 254a.
    98. Lupashin V.V, Suvorova E.S. Characterization of the Golgi tethering factor Sec34p. 28th Steenbock Symposium. 2000; 74.
    99. Lupashin V.V, Suvorova E.S, Kleve A.R. Characterization of the Golgi tethering factor Sec34p in yeast and human cells. 2000; 11S: 209a-210a.
    100. VanRheenen SM, Cao X, Sapperstein SK, Chiang EC, Lupashin VV, Barlowe C, Waters MG. Sec34p, a protein required for vesicle tethering to the yeast Golgi apparatus, is in a complex with Sec35p. J Cell Biol. 1999 Nov 15; 147(4):729-42. PMID: 10562277.
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    101. Kononova S, Sitnikova V, Lupashin V.V. Study of membrane traffic at the yeast cell surface: in vivo and in vitro approaches. Sixth ASCB/EMBO Joint Meeting. 1999.
    102. VanRheenen SM, Cao X, Lupashin VV, Barlowe C, Waters MG. Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking. J Cell Biol. 1998 Jun 01; 141(5):1107-19. PMID: 9606204.
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    103. Lupashin VV, Pokrovskaya ID, McNew JA, Waters MG. Characterization of a novel yeast SNARE protein implicated in Golgi retrograde traffic. Mol Biol Cell. 1997 Dec; 8(12):2659-76. PMID: 9398683.
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    104. Lupashin VV, Waters MG. t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase. Science. 1997 May 23; 276(5316):1255-8. PMID: 9157884.
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    105. Lupashin V.V, Van Rheenen S.M, Waters M.G. Regulation of vesicle docking in the secretory pathway. 1997; 13(SI), S24.
    106. Sapperstein SK, Lupashin VV, Schmitt HD, Waters MG. Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol. 1996 Mar; 132(5):755-67. PMID: 8603910.
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    107. Lupashin VV, Hamamoto S, Schekman RW. Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes. J Cell Biol. 1996 Feb; 132(3):277-89. PMID: 8636207.
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    108. VanRheenen S.M, Lupashin V.V, Waters M.G. Identification and characterization of novel proteins involved in the regulation of SNARE-complex assembly. 1996; 420-420 Suppl S.
    109. Lupashin V.V, Waters M.G. In vivo and in vitro analysis of ER to Golgi SNARE complex assembly in yeast. 1996; 421-421 Suppl. S.
    110. Lupashin V.V, Sapperstein S.K, Waters M.G. Uso1p is required for assembly of the ER to Golgi SNARE complex. 1995; 6:290A.
    111. Lupashin V, Hamamoto S, Schekman R.W. In-vitro reconstitution of targeting and fusion of ER-derived vesicles with Golgi membranes in yeast. 1995; (6) A1377-A1377.
    112. Tsiomenko A.V, Tujmetova G.P, Lupashin V.V, Karpychev I.V, Eldarov M.A, Skryabin K.G, Kulaev I.S. The role of glycosylation in protein secretion. 7th Soviet-Italian Symposium. 1993.
    113. Tsiomenko A.B, Tujmetova G.P, Lupashin V.V, Karpychev I.V, Eldarov M.A, Skryabin K.G, Kulaev I.S. Macromolecules in functioning cell. The role of glycosylation in protein secretion in yeast. 1993; (1993):135-142.
    114. Tsiomenko A.B, Kononova S.V, Lupashin V.V, Tujmetova G.P, Gulaymova D.E, Ratner Ye.N. Localization and characterization of yeast secretory proteins stimulated by heat shock. ISSY 16th. 1993.
    115. Lupashin VV, Kononova SV, Tsiomenko AB, Kulaev IS. Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock. Yeast. 1992 Mar; 8(3):157-69. PMID: 1574924.
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    116. Ushakov V.M, Tsiomenko A.B, Tujmetova G.P, Lupashin V.V, Kulaev I.S. The way for invertase isolation from the yeast cells in process of cultivation. Biotechnologiya. 1992; 1, 43-45.
    117. Ushakov V.M, Tsiomenko A.B, Tujmetova G.P, Lupashin V.V. Method of extracting invertase from yeast cells during their culturing. Soviet Biothechnology c/c of Biotechnologiya. 1992; 1, 58.
    118. Tsiomenko A, Musaev D, Lupashin V, Kulaev I. Secreted alpha-amylase of the basidiomycetous yeast Filobasidium capsuligenum - isolation, purification and properties. Biochemistry-USSR. 1992; 57:297-303.
    119. Lupashin VV, Karpychev IV, El'darov MA, Tsiomenko AB, Skriabin KG, Kulaev IS. [Localization of acid phosphatase in Saccharomyces cerevisiae and its export into culture media depends on the type of the N-terminal signal peptide]. Mol Gen Mikrobiol Virusol. 1991 Jun; (6):16-8. PMID: 1944322.
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    120. Lupashin VV, Kononova SV, Ratner EN, Tsiomenko AB, Kulaev IS. [A new secreted protein from Saccharomyces cerevisiae, stimulated by heat shock]. Dokl Akad Nauk SSSR. 1991; 317(5):1257-60. PMID: 1743089.
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    121. Tujmetova G.P, Karpychev I. V, Eldarov M.A, Lupashin V.V, Skryabin K.G, Tsiomenko A.B. S.cerevisiae alpha-factor prosegment is essential for export of human growth hormone into culture medium. 1991; 144.
    122. Tsiomenko AB, Lupashin VV, Morzunov SA, Karpychev IV, El'darov MA, Bezhelarskaia SN, Rubtsov PM, Skriabin KG, Kulaev IS. [The nature of N-terminal signal sequence determines the type of intracellular distribution and effectiveness of export of human growth hormone in Saccharomyces cerevisiae]. Mol Biol (Mosk). 1990 Jul-Aug; 24(4):1126-33. PMID: 2250679.
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    123. Kulaev I.S, Tsiomenko A.B, Tujmetova G.P, Lupashin V.V, Karpychev I.V, Eldarov M.A, Skryabin K.G. Glycosylation of secreted proteins in yeast. 7th Int'l Symposium. 1990; 41.
    124. Lupashin V.V, Stavitskaya Y.E, Karpychev I.V, Eldarov M.A, Tsiomenko A.B. Optimal conditions for homologic and heterologic protein export by S. cerevisiae. 14th Int'l Congress of Biochemistry, v.2. 1988; 70.
    125. Tsiomenko AB, Lupashin VV, Dmitriev VV, Kulaev IS. [Cell wall permeability and export of proteins into the culture broth of Saccharomyces cerevisiae]. Mikrobiologiia. 1987 Sep-Oct; 56(5):797-804. PMID: 3329283.
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    126. Skriabin K.G, Tsiomenko A.B, Morzunov S.P, Karpychev I.V, Lupashin V.V, Eldarov M.A, Rubtzov P.M, Kulaev I.S, Baev A.A. Synthesis and secretion of human growth hormone in the yeast S. cerevisiae. Biotekhnologiya. 1987; 153:163-168.
    127. Vagabov V.M, Knyazeva I.N, Lupashin V.V, Krupyanko V.I, Kulaev I.S. Study of chitin-synthase from the yeast Saccharomyces carlsbergensis. Effect of ATP on solubilized enzyme. Biokhimiya. 1987; 52:534-542.
    128. Tsiomenko A.B, Lupashin V.V, Kulaev I.S. Extracellular enzymes of microorganisms. Export of proteins into the cultural media by the yeast S. cerevisiae. 1987; (1987):205-208.
    129. Vagabov V.M, Lupashin V.V, Knyazeva I.N, Kulaev I.S. Study of the Influence of ATP on the activity of membrane-bound chitin synthase of the yeast Saccharomyces carlsbergensis. Biokhimiya. 1986; 51:1802-1810.
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