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Search Results to Kevin Raney

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overview My laboratory is interested in the enzymology and chemistry of nucleic acid enzymes. Helicases are enzymes that manipulate DNA and RNA in all aspects of nucleic acid metabolism. We are studying a DNA helicase from Bacteriophage T4 called Dda (for DNA-Dependent-ATPase) in order to develop a detailed chemical and kinetic mechanism for DNA unwinding by this model DNA helicase. A second enzyme we study is called Pif1. This helicase is involved in many aspects of DNA metabolism ranging from telomere maintenance to transcription. Pif1 binds tightly to unusual DNA structures called quadruplexes, for which the biological functions are being intensively explored. A second project involves the Hepatitis C viral helicase NS3 (Non-Structural Protein 3). NS3 is an RNA helicase that is capable of unwinding DNA. We are studying the mechanism of NS3 as well as its interactions with other HCV and cellular proteins. Our goal is to recapitulate RNA replication in vitro using biological relevant substrates and proteins and a biologically relevant sub-genomic replicon of HCV. Our research projects are currently expanding in development of new tools for studying and protein-protein interactions and post-translational modifications at specific sites in the genome using a Crispr-based approach coupled with protein mass spectrometry. In a new project, we have recently discovered a possible signaling mechanism by which cells respond to DNA damage. During oxidative stress, guanine residues are oxidized, leading to excision of the damaged DNA. When the excised DNA consists of specific sequences containing runs of guanine, the resulting DNA fragment can fold into a stable structure called quadruplex DNA. Telomeric DNA is particularly susceptible to oxidative stress and contains sequences that readily fold into quadruplex structures. The excised DNA quadruplexes can bind to proteins such as DHX36 (a helicase), leading to formation of sub-organelles called stress granules. The functional role of stress granules is to modulate translation. Hence, this mechanism provides a stepwise chemical mechanism for the cell to respond to DNA damage leading to changes in translation.

One or more keywords matched the following items that are connected to Raney, Kevin

Item TypeName
Academic Article Multiple full-length NS3 molecules are required for optimal unwinding of oligonucleotide DNA in vitro.
Academic Article Structural and biological identification of residues on the surface of NS3 helicase required for optimal replication of the hepatitis C virus.
Academic Article NS3 helicase from the hepatitis C virus can function as a monomer or oligomer depending on enzyme and substrate concentrations.
Academic Article Helicase-catalysed translocation and strand separation.
Academic Article Hepatitis C virus nonstructural protein 5A (NS5A) is an RNA-binding protein.
Academic Article Phosphate release contributes to the rate-limiting step for unwinding by an RNA helicase.
Academic Article Mechanisms: molecular machines.
Academic Article RNA unwinding activity of the hepatitis C virus NS3 helicase is modulated by the NS5B polymerase.
Academic Article Superfamily 2 helicases.
Academic Article Modulation of the hepatitis C virus RNA-dependent RNA polymerase activity by the non-structural (NS) 3 helicase and the NS4B membrane protein.
Academic Article Hepatitis C virus nonstructural protein 5A: biochemical characterization of a novel structural class of RNA-binding proteins.
Academic Article Unwinding of unnatural substrates by a DNA helicase.
Academic Article Protein displacement by helicases.
Academic Article Unwinding of nucleic acids by HCV NS3 helicase is sensitive to the structure of the duplex.
Concept RNA, Fungal
Concept RNA, Viral
Concept RNA, Guide
Concept RNA
Concept DNA-Directed RNA Polymerases
Concept RNA Viruses
Concept RNA Helicases
Concept RNA, Messenger
Concept DEAD-box RNA Helicases
Concept RNA-Binding Proteins
Concept RNA, Double-Stranded
Concept RNA, Catalytic
Academic Article Mutation in the Putative Nuclear Localization Sequence of the Hepatitis C NS3 Protein Reduce Colony Formation After Transfection of HCV Replicon RNA into Huh 7 Cell
Academic Article Peptide Nucleic Acid Inhibits HCV-Ns3 Catalyzed Unwinding of DNA and RNA Substrates
Academic Article "RNA Helicases"
Academic Article Identification of Protein Surfaces of NS3 that are Required for HCV Replication
Academic Article Mutations in the Putative Nuclear Localization Sequence of the Hepatitis C NS3 Protein Conoly Formation after Transfection of HCV Replicon RNA into Huh 7 Cells
Academic Article A CRISPR-based approach for proteomic analysis of a single genomic locus.
Academic Article Probing RNA translocases with DNA.
Academic Article Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes.
Academic Article The Yeast Mitochondrial RNA Polymerase and Transcription Factor Complex Catalyzes Efficient Priming of DNA Synthesis on Single-stranded DNA.
Academic Article Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress.
Academic Article N-Naphthoyl-substituted indole thio-barbituric acid analogs inhibit the helicase activity of the hepatitis C virus NS3.
Grant Mechanisms of RNA binding and remodeling proteins
Grant HCV NS3: Biological, Biochemical and Structural Analysis
Grant HCV NS3 and NS5A: Biochemical Mechanisms and Biological Functions
Grant Functions and Mechanisms of Helicases and G-Quadruplex Nucleic Acids
Grant DNA Helicases: Mechanism and Function
Academic Article DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA.
Academic Article Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays.
Academic Article G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.
Academic Article RNA helicases required for viral propagation in humans.

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