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Robert Eoff

TitleAssociate Professor
InstitutionUniversity of Arkansas for Medical Sciences
DepartmentBiochemistry & Molecular Biology, College of Medicine
DivisionBiochem & Molecular Y-Family DNA
AddressB421E Biomedical Research I
325 S. Elm St.
Mail Slot # 516
Little Rock AR 72205
Phone501-686-8343
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    Collapse Overview 
    Collapse overview
    The research I pursue is focused on DNA replication and seeks to provide fundamental insights into how these mechanisms are related to human health, especially cancer. I have expertise in a number of areas pertinent to the current application including: biochemical/biophysical analysis of enzymes, structural biology (X-ray crystallography and molecular modeling techniques), mass spectrometry and genomic instability in cancer. In addition to in silico modeling approaches, I have been involved in solving thirty-four crystal structures submitted to the Protein Data Bank. I am first author on seventeen of these PDB submissions, and my laboratory has solved five crystal structures since I joined the faculty at UAMS. I have an active collaboration (General users proposal, GUP-41183) with the Northeastern collaborative access team (NE-CAT) at the Advanced Photon Source (APS) that grants us access to the 24-ID-E and 24-ID-C beamlines for data collection. The research I pursue is focused on DNA damage tolerance pathways and seeks to provide fundamental insights into how these mechanisms function under basal conditions and how they go astray during tumorigenesis or as a function of age. I have received funding from the National Institutes of Health in the form of an R00 award (GM084460) and a R01 (CA183895). I have established a strong research program with two senior post-doctoral fellows and three Ph.D. students. In less than four years, my team has published nine full, peer-reviewed research articles on which I am corresponding author. Work from my group has recently culminated in a manuscript that reveals important and previously unrecognized properties related to Werner’s syndrome protein (WRN) modulation of polymerase activity during bypass of oxidative DNA damage. Experiments from my group illustrate that WRN has a strong impact on the DNA adduct bypass properties of human DNA polymerases (pols) eta and kappa, inducing more accurate synthesis across oxidative damage (published in The Journal of Biological Chemistry and Nucleic Acids Research). Another study from my laboratory that was conducted in collaboration with Prof. Peter Crooks (UAMS) identified novel small-molecule inhibitors of the replication stress response enzyme human DNA polymerase eta, with the resulting manuscript recently being published in ACS Chemical Biology. We continue to pursue projects related to translesion polymerase activity in cancer and are actively developing new TLS pol inhibitors as a way to sensitize tumors to genotoxic anti-cancer drugs (e.g. cisplatin, doxorubicin). My long-term goal is to contribute in a meaningful fashion to the scientific endeavors that seek to improve human health, our ability to treat disease & our fundamental understanding of living systems.


    Collapse Affiliation 
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    American Society of Biochemistry and Molecular Biology

    Collapse Research Funds 
    Collapse research activities
    1903357     (EOFF, ROBERT)Aug 1, 2019 - Jul 31, 2023
    National Science Foundation
    Replication of G-quadruplex DNA by translesion polymerases
    Role: Principal Investigator

    R35GM122601     (RANEY, KEVIN D)May 1, 2017 - Apr 30, 2022
    NIH/NIGMS
    Functions and Mechanisms of Helicases and G-Quadruplex Nucleic Acids
    Role: Co-Investigator

    R01GM117439     (RANEY, KEVIN D)Sep 21, 2015 - Jun 30, 2019
    NIH/NIGMS
    G-quadruplex DNA as a chemical signaling agent
    Role: Co-Investigator

    R01CA183895     (EOFF, ROBERT L)Apr 4, 2014 - Feb 28, 2019
    NIH/NCI
    Translesion DNA polymerase kappa activity in gliomas
    Role: Principal Investigator

    R00GM084460     (EOFF, ROBERT L)Jul 1, 2009 - Dec 31, 2013
    NIH/NIGMS
    Coordinating Translesion DNA Synthesis Opposite Damaged DNA
    Role: Principal Investigator

    K99GM084460     (EOFF, ROBERT L)Jul 1, 2009 - Jun 30, 2011
    NIH/NIGMS
    Coordinating Translesion DNA Synthesis Opposite Damaged DNA
    Role: Principal Investigator

    F32CA119776     (EOFF, ROBERT L)Aug 1, 2006 - Jul 31, 2009
    NIH/NCI
    Translesion Synthesis Opposite Carcinogen Bound DNA
    Role: Principal Investigator

    Collapse Outreach 

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions. Don't see publications published under other names? Login to add alternative names.
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    1. West KL, Kelliher JL, Xu Z, An L, Reed MR, Eoff RL, Wang J, Huen MSY, Leung JWC. LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation. Nucleic Acids Res. 2019 07 09; 47(12):6236-6249. PMID: 30982887.
      View in: PubMed
    2. Ketkar A, Maddukuri L, Penthala NR, Reed MR, Zafar MK, Crooks PA, Eoff RL. Inhibition of Human DNA Polymerases Eta and Kappa by Indole-Derived Molecules Occurs through Distinct Mechanisms. ACS Chem Biol. 2019 06 21; 14(6):1337-1351. PMID: 31082191.
      View in: PubMed
    3. Zafar MK, Maddukuri L, Ketkar A, Penthala NR, Reed MR, Eddy S, Crooks PA, Eoff RL. A Small-Molecule Inhibitor of Human DNA Polymerase ? Potentiates the Effects of Cisplatin in Tumor Cells. Biochemistry. 2018 02 20; 57(7):1262-1273. PMID: 29345908.
      View in: PubMed
    4. Madadi NR, Penthala NR, Ketkar A, Eoff RL, Trujullo-Alonso V, Guzman ML, Crooks PA. Synthesis and Evaluation of 2-Naphthaleno trans-Stilbenes and Cyanostilbenes as Anticancer Agents. Anticancer Agents Med Chem. 2018; 18(4):556-564. PMID: 28403783.
      View in: PubMed
    5. Zafar MK, Eoff RL. Translesion DNA Synthesis in Cancer: Molecular Mechanisms and Therapeutic Opportunities. Chem Res Toxicol. 2017 11 20; 30(11):1942-1955. PMID: 28841374.
      View in: PubMed
    6. Eoff RL, Raney KD. A catch and release program for single-stranded DNA. J Biol Chem. 2017 08 04; 292(31):13085-13086. PMID: 28778884.
      View in: PubMed
    7. Ketkar A, Voehler M, Mukiza T, Eoff RL. Residues in the RecQ C-terminal Domain of the Human Werner Syndrome Helicase Are Involved in Unwinding G-quadruplex DNA. J Biol Chem. 2017 02 24; 292(8):3154-3163. PMID: 28069813.
      View in: PubMed
    8. Yadlapalli JSK, Ford BM, Ketkar A, Wan A, Penthala NR, Eoff RL, Prather PL, Dobretsov M, Crooks PA. Antinociceptive effects of the 6-O-sulfate ester of morphine in normal and diabetic rats: Comparative role of mu- and delta-opioid receptors. Pharmacol Res. 2016 11; 113(Pt A):335-347. PMID: 27637375.
      View in: PubMed
    9. Eddy S, Tillman M, Maddukuri L, Ketkar A, Zafar MK, Eoff RL. Human Translesion Polymerase ? Exhibits Enhanced Activity and Reduced Fidelity Two Nucleotides from G-Quadruplex DNA. Biochemistry. 2016 09 20; 55(37):5218-29. PMID: 27525498.
      View in: PubMed
    10. Bostian AC, Eoff RL. Aberrant Kynurenine Signaling Modulates DNA Replication Stress Factors and Promotes Genomic Instability in Gliomas. Chem Res Toxicol. 2016 09 19; 29(9):1369-80. PMID: 27482758.
      View in: PubMed
    11. Byrd AK, Zybailov BL, Maddukuri L, Gao J, Marecki JC, Jaiswal M, Bell MR, Griffin WC, Reed MR, Chib S, Mackintosh SG, MacNicol AM, Baldini G, Eoff RL, Raney KD. Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress. J Biol Chem. 2016 08 19; 291(34):18041-57. PMID: 27369081.
      View in: PubMed
    12. Madadi NR, Ketkar A, Penthala NR, Bostian AC, Eoff RL, Crooks PA. Dioxol and dihydrodioxin analogs of 2- and 3-phenylacetonitriles as potent anti-cancer agents with nanomolar activity against a variety of human cancer cells. Bioorg Med Chem Lett. 2016 May 01; 26(9):2164-9. PMID: 27017113.
      View in: PubMed
    13. Yeom M, Kim IH, Kim JK, Kang K, Eoff RL, Guengerich FP, Choi JY. Effects of Twelve Germline Missense Variations on DNA Lesion and G-Quadruplex Bypass Activities of Human DNA Polymerase REV1. Chem Res Toxicol. 2016 Mar 21; 29(3):367-79. PMID: 26914252.
      View in: PubMed
    14. Bostian AC, Maddukuri L, Reed MR, Savenka T, Hartman JH, Davis L, Pouncey DL, Miller GP, Eoff RL. Kynurenine Signaling Increases DNA Polymerase Kappa Expression and Promotes Genomic Instability in Glioblastoma Cells. Chem Res Toxicol. 2016 Jan 19; 29(1):101-8. PMID: 26651356.
      View in: PubMed
    15. Penthala NR, Ketkar A, Sekhar KR, Freeman ML, Eoff RL, Balusu R, Crooks PA. 1-Benzyl-2-methyl-3-indolylmethylene barbituric acid derivatives: Anti-cancer agents that target nucleophosmin 1 (NPM1). Bioorg Med Chem. 2015 Nov 15; 23(22):7226-33. PMID: 26602084.
      View in: PubMed
    16. Madadi NR, Penthala NR, Howk K, Ketkar A, Eoff RL, Borrelli MJ, Crooks PA. Synthesis and biological evaluation of novel 4,5-disubstituted 2H-1,2,3-triazoles as cis-constrained analogues of combretastatin A-4. Eur J Med Chem. 2015 Oct 20; 103:123-32. PMID: 26352674.
      View in: PubMed
    17. Penthala NR, Madhukuri L, Thakkar S, Madadi NR, Lamture G, Eoff RL, Crooks PA. Synthesis and anti-cancer screening of novel heterocyclic-(2H)-1,2,3-triazoles as potential anti-cancer agents. Medchemcomm. 2015 Aug 01; 6(8):1535-1543. PMID: 27066215.
      View in: PubMed
    18. Eddy S, Maddukuri L, Ketkar A, Zafar MK, Henninger EE, Pursell ZF, Eoff RL. Evidence for the kinetic partitioning of polymerase activity on G-quadruplex DNA. Biochemistry. 2015 May 26; 54(20):3218-30. PMID: 25903680.
      View in: PubMed
    19. Madadi NR, Zong H, Ketkar A, Zheng C, Penthala NR, Janganati V, Bommagani S, Eoff RL, Guzman ML, Crooks PA. Synthesis and evaluation of a series of resveratrol analogues as potent anti-cancer agents that target tubulin. Medchemcomm. 2015 May 01; 6(3):788-794. PMID: 26257861.
      View in: PubMed
    20. Penthala NR, Zong H, Ketkar A, Madadi NR, Janganati V, Eoff RL, Guzman ML, Crooks PA. Synthesis, anticancer activity and molecular docking studies on a series of heterocyclic trans-cyanocombretastatin analogues as antitubulin agents. Eur J Med Chem. 2015 Mar 06; 92:212-20. PMID: 25557492.
      View in: PubMed
    21. Zafar MK, Ketkar A, Lodeiro MF, Cameron CE, Eoff RL. Kinetic analysis of human PrimPol DNA polymerase activity reveals a generally error-prone enzyme capable of accurately bypassing 7,8-dihydro-8-oxo-2'-deoxyguanosine. Biochemistry. 2014 Oct 21; 53(41):6584-94. PMID: 25255211.
      View in: PubMed
    22. Maddukuri L, Ketkar A, Eddy S, Zafar MK, Eoff RL. The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DNA polymerase kappa. Nucleic Acids Res. 2014 Oct 29; 42(19):12027-40. PMID: 25294835.
      View in: PubMed
    23. Kim J, Song I, Jo A, Shin JH, Cho H, Eoff RL, Guengerich FP, Choi JY. Biochemical analysis of six genetic variants of error-prone human DNA polymerase ? involved in translesion DNA synthesis. Chem Res Toxicol. 2014 Oct 20; 27(10):1837-52. PMID: 25162224.
      View in: PubMed
    24. Zhao L, Pence MG, Eoff RL, Yuan S, Fercu CA, Guengerich FP. Elucidation of kinetic mechanisms of human translesion DNA polymerase ? using tryptophan mutants. FEBS J. 2014 Oct; 281(19):4394-410. PMID: 25065501.
      View in: PubMed
    25. Eddy S, Ketkar A, Zafar MK, Maddukuri L, Choi JY, Eoff RL. Human Rev1 polymerase disrupts G-quadruplex DNA. Nucleic Acids Res. 2014 Mar; 42(5):3272-85. PMID: 24366879.
      View in: PubMed
    26. Maddukuri L, Shuck SC, Eoff RL, Zhao L, Rizzo CJ, Guengerich FP, Marnett LJ. Replication, repair, and translesion polymerase bypass of N6-oxopropenyl-2'-deoxyadenosine. Biochemistry. 2013 Dec 03; 52(48):8766-76. PMID: 24171480.
      View in: PubMed
    27. Schaetzlein S, Chahwan R, Avdievich E, Roa S, Wei K, Eoff RL, Sellers RS, Clark AB, Kunkel TA, Scharff MD, Edelmann W. Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes. Proc Natl Acad Sci U S A. 2013 Jul 02; 110(27):E2470-9. PMID: 23754438.
      View in: PubMed
    28. Coggins GE, Maddukuri L, Penthala NR, Hartman JH, Eddy S, Ketkar A, Crooks PA, Eoff RL. N-Aroyl indole thiobarbituric acids as inhibitors of DNA repair and replication stress response polymerases. ACS Chem Biol. 2013 Aug 16; 8(8):1722-9. PMID: 23679919.
      View in: PubMed
    29. Ketkar A, Zafar MK, Maddukuri L, Yamanaka K, Banerjee S, Egli M, Choi JY, Lloyd RS, Eoff RL. Leukotriene biosynthesis inhibitor MK886 impedes DNA polymerase activity. Chem Res Toxicol. 2013 Feb 18; 26(2):221-32. PMID: 23305233.
      View in: PubMed
    30. Ketkar A, Zafar MK, Banerjee S, Marquez VE, Egli M, Eoff RL. Differential furanose selection in the active sites of archaeal DNA polymerases probed by fixed-conformation nucleotide analogues. Biochemistry. 2012 Nov 13; 51(45):9234-44. PMID: 23050956.
      View in: PubMed
    31. Maddukuri L, Ketkar A, Eddy S, Zafar MK, Griffin WC, Eoff RL. Enhancement of human DNA polymerase ? activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein. J Biol Chem. 2012 Dec 07; 287(50):42312-23. PMID: 23045531.
      View in: PubMed
    32. Yamanaka K, Dorjsuren D, Eoff RL, Egli M, Maloney DJ, Jadhav A, Simeonov A, Lloyd RS. A comprehensive strategy to discover inhibitors of the translesion synthesis DNA polymerase ?. PLoS One. 2012; 7(10):e45032. PMID: 23056190.
      View in: PubMed
    33. Ketkar A, Zafar MK, Banerjee S, Marquez VE, Egli M, Eoff RL. A nucleotide-analogue-induced gain of function corrects the error-prone nature of human DNA polymerase iota. J Am Chem Soc. 2012 Jun 27; 134(25):10698-705. PMID: 22632140.
      View in: PubMed
    34. Adedeji AO, Marchand B, Te Velthuis AJ, Snijder EJ, Weiss S, Eoff RL, Singh K, Sarafianos SG. Mechanism of nucleic acid unwinding by SARS-CoV helicase. PLoS One. 2012; 7(5):e36521. PMID: 22615777.
      View in: PubMed
    35. Choi JY, Eoff RL, Pence MG, Wang J, Martin MV, Kim EJ, Folkmann LM, Guengerich FP. Roles of the four DNA polymerases of the crenarchaeon Sulfolobus solfataricus and accessory proteins in DNA replication. J Biol Chem. 2011 Sep 09; 286(36):31180-93. PMID: 21784862.
      View in: PubMed
    36. Eoff RL, McGrath CE, Maddukuri L, Salamanca-Pinzón SG, Marquez VE, Marnett LJ, Guengerich FP, Egli M. Selective modulation of DNA polymerase activity by fixed-conformation nucleoside analogues. Angew Chem Int Ed Engl. 2010 Oct 04; 49(41):7481-5. PMID: 20814997.
      View in: PubMed
    37. Maddukuri L, Eoff RL, Choi JY, Rizzo CJ, Guengerich FP, Marnett LJ. In vitro bypass of the major malondialdehyde- and base propenal-derived DNA adduct by human Y-family DNA polymerases ?, ?, and Rev1. Biochemistry. 2010 Sep 28; 49(38):8415-24. PMID: 20726503.
      View in: PubMed
    38. Eoff RL, Choi JY, Guengerich FP. Mechanistic Studies with DNA Polymerases Reveal Complex Outcomes following Bypass of DNA Damage. J Nucleic Acids. 2010 Sep 26; 2010. PMID: 20936119.
      View in: PubMed
    39. Irimia A, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M. Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 01; 66(Pt 9):1013-8. PMID: 20823515.
      View in: PubMed
    40. Eoff RL, Raney KD. Kinetic mechanism for DNA unwinding by multiple molecules of Dda helicase aligned on DNA. Biochemistry. 2010 Jun 01; 49(21):4543-53. PMID: 20408588.
      View in: PubMed
    41. Choi J-Y, Eoff RL, Guengerich FP. Springer, New York, USA: Trevor Penning; Chemical Carcinogenesis: Bypass DNA polymerases. Chemical Carcinogenesis: Bypass DNA polymerases. 2010.
    42. Eoff RL, Egli M, Guengerich FP. Wiley-VCH, Weinheim, Germany: Geacintov, N. E. & Broyde, S. (eds); 2010. Impact of Chemical Adducts on Translesion Synthesis in Replicative and Bypass DNA Polymerases: From Structure to Function. Impact of Chemical Adducts on Translesion Synthesis in Replicative and Bypass DNA Polymerases: From Structure to Function. 2010.
    43. Eoff RL, Stafford JB, Szekely J, Rizzo CJ, Egli M, Guengerich FP, Marnett LJ. Structural and functional analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed bypass of the malondialdehyde-deoxyguanosine adduct. Biochemistry. 2009 Aug 04; 48(30):7079-88. PMID: 19492857.
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    44. Zhang H, Eoff RL, Kozekov ID, Rizzo CJ, Egli M, Guengerich FP. Structure-function relationships in miscoding by Sulfolobus solfataricus DNA polymerase Dpo4: guanine N2,N2-dimethyl substitution produces inactive and miscoding polymerase complexes. J Biol Chem. 2009 Jun 26; 284(26):17687-99. PMID: 19542237.
      View in: PubMed
    45. Irimia A, Eoff RL, Guengerich FP, Egli M. Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct. J Biol Chem. 2009 Aug 14; 284(33):22467-80. PMID: 19542228.
      View in: PubMed
    46. Eoff RL, Sanchez-Ponce R, Guengerich FP. Conformational changes during nucleotide selection by Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2009 Jul 31; 284(31):21090-9. PMID: 19515847.
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    47. Voehler MW, Eoff RL, McDonald WH, Guengerich FP, Stone MP. Modulation of the structure, catalytic activity, and fidelity of African swine fever virus DNA polymerase X by a reversible disulfide switch. J Biol Chem. 2009 Jul 03; 284(27):18434-44. PMID: 19419958.
      View in: PubMed
    48. Choi JY, Lim S, Eoff RL, Guengerich FP. Kinetic analysis of base-pairing preference for nucleotide incorporation opposite template pyrimidines by human DNA polymerase iota. J Mol Biol. 2009 Jun 05; 389(2):264-74. PMID: 19376129.
      View in: PubMed
    49. Pallan PS, Prakash TP, Li F, Eoff RL, Manoharan M, Egli M. A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution. Chem Commun (Camb). 2009 Apr 21; (15):2017-9. PMID: 19333476.
      View in: PubMed
    50. Stafford JB, Eoff RL, Kozekova A, Rizzo CJ, Guengerich FP, Marnett LJ. Translesion DNA synthesis by human DNA polymerase eta on templates containing a pyrimidopurinone deoxyguanosine adduct, 3-(2'-deoxy-beta-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one. Biochemistry. 2009 Jan 20; 48(2):471-80. PMID: 19108641.
      View in: PubMed
    51. Zhang H, Eoff RL, Kozekov ID, Rizzo CJ, Egli M, Guengerich FP. Versatility of Y-family Sulfolobus solfataricus DNA polymerase Dpo4 in translesion synthesis past bulky N2-alkylguanine adducts. J Biol Chem. 2009 Feb 06; 284(6):3563-76. PMID: 19059910.
      View in: PubMed
    52. Isin EM, Sohl CD, Eoff RL, Guengerich FP. Cooperativity of cytochrome P450 1A2: interactions of 1,4-phenylene diisocyanide and 1-isopropoxy-4-nitrobenzene. Arch Biochem Biophys. 2008 May 01; 473(1):69-75. PMID: 18328798.
      View in: PubMed
    53. Sohl CD, Isin EM, Eoff RL, Marsch GA, Stec DF, Guengerich FP. Cooperativity in oxidation reactions catalyzed by cytochrome P450 1A2: highly cooperative pyrene hydroxylation and multiphasic kinetics of ligand binding. J Biol Chem. 2008 Mar 14; 283(11):7293-308. PMID: 18187423.
      View in: PubMed
    54. Irimia A, Eoff RL, Pallan PS, Guengerich FP, Egli M. Structure and activity of Y-class DNA polymerase DPO4 from Sulfolobus solfataricus with templates containing the hydrophobic thymine analog 2,4-difluorotoluene. J Biol Chem. 2007 Dec 14; 282(50):36421-33. PMID: 17951245.
      View in: PubMed
    55. Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP. Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2007 Jul 06; 282(27):19831-43. PMID: 17468100.
      View in: PubMed
    56. Eoff RL, Angel KC, Egli M, Guengerich FP. Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. J Biol Chem. 2007 May 04; 282(18):13573-84. PMID: 17337730.
      View in: PubMed
    57. Eoff RL, Irimia A, Egli M, Guengerich FP. Sulfolobus solfataricus DNA polymerase Dpo4 is partially inhibited by "wobble" pairing between O6-methylguanine and cytosine, but accurate bypass is preferred. J Biol Chem. 2007 Jan 12; 282(2):1456-67. PMID: 17105728.
      View in: PubMed
    58. Sikora B, Eoff RL, Matson SW, Raney KD. DNA unwinding by Escherichia coli DNA helicase I (TraI) provides evidence for a processive monomeric molecular motor. J Biol Chem. 2006 Nov 24; 281(47):36110-6. PMID: 16984922.
      View in: PubMed
    59. Irimia A, Zang H, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M. Calcium is a cofactor of polymerization but inhibits pyrophosphorolysis by the Sulfolobus solfataricus DNA polymerase Dpo4. Biochemistry. 2006 May 16; 45(19):5949-56. PMID: 16681366.
      View in: PubMed
    60. Eoff RL, Raney KD. Intermediates revealed in the kinetic mechanism for DNA unwinding by a monomeric helicase. Nat Struct Mol Biol. 2006 Mar; 13(3):242-9. PMID: 16474403.
      View in: PubMed
    61. Spurling TL, Eoff RL, Raney KD. Dda helicase unwinds a DNA-PNA chimeric substrate: evidence for an inchworm mechanism. Bioorg Med Chem Lett. 2006 Apr 01; 16(7):1816-20. PMID: 16439125.
      View in: PubMed
    62. Eoff RL, Raney KD. Helicase-catalysed translocation and strand separation. Biochem Soc Trans. 2005 Dec; 33(Pt 6):1474-8. PMID: 16246149.
      View in: PubMed
    63. Eoff RL, Spurling TL, Raney KD. Chemically modified DNA substrates implicate the importance of electrostatic interactions for DNA unwinding by Dda helicase. Biochemistry. 2005 Jan 18; 44(2):666-74. PMID: 15641792.
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    64. Nanduri B, Byrd AK, Eoff RL, Tackett AJ, Raney KD. Pre-steady-state DNA unwinding by bacteriophage T4 Dda helicase reveals a monomeric molecular motor. Proc Natl Acad Sci U S A. 2002 Nov 12; 99(23):14722-7. PMID: 12411580.
      View in: PubMed
    65. Nanduri B, Eoff RL, Tackett AJ, Raney KD. Measurement of steady-state kinetic parameters for DNA unwinding by the bacteriophage T4 Dda helicase: use of peptide nucleic acids to trap single-stranded DNA products of helicase reactions. Nucleic Acids Res. 2001 Jul 01; 29(13):2829-35. PMID: 11433029.
      View in: PubMed
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