Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves.
Boyanton BL, Almradi A, Espy MJ, Prada AE, Gibson JP, Pritt BS. Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves. J Clin Microbiol. 2014 Mar; 52(3):957-9.